Stability-Indicating Quantification of Ciprofloxacin in the Presence of Its Main Photo-Degradation Product by CZE and UPLC: A Comparative Study

Active pharmaceutical ingredients (APIs) can be assured to safe and effective with the help of stability-indicating procedures. An accurate comparison comprising utilization capillary zone electrophoresis (CZE) ultra-performance liquid chromatography (UPLC) for sensitive measurement ciprofloxacin (CPF) in presence its major photo-degradation product was conducted. The CZE UPLC working conditions were optimized obtain best pattern separation CPF photo-degradant. linearity range cited techniques confirmed 0.5 50 µg/mL. A thorough validation scheme according ICH-Q2B criteria performed, including linearity, accuracy, precision, robustness, detection, quantification limits. Selective up 90% main photo-degradant carried out using proposed methods. For analysis tablet intravenous (I.V.) solution forms, procedures applied. suggested methods applied keep an eye on safety efficacy either bulk or dosage forms.